Bioremediation of PFAS with special bacteria: Labrys portucalensis F11 [0.6.1 |0.6.15], Acidimicrobium sp. strain A6, and certain Pseudomonas species

https://www.pca.state.mn.us/pollutants-and-contaminants/pfas 

   On a per-pound basis, this type of clean up would cost $2.8 million-$18 million per pound of PFAS. In comparison, purchasing PFAS costs between $50-$1,000 per pound.

https://www.buffalo.edu/news/releases/2025/01/bacteria-found-to-eat-forever-chemicals.html

Now, a University at Buffalo-led team has identified a strain of bacteria that can break down and transform at least three types of PFAS, and, perhaps even more crucially, some of the toxic byproducts of the bond-breaking process.

Published in this month’s issue of Science of the Total Environment, the team’s study found that Labrys portucalensis F11 (F11) metabolized over 90% of perfluorooctane sulfonic acid (PFOS) following an exposure period of 100 days....

Eventually, F11 could be deployed in PFAS-contaminated water and soil. This might involve creating conditions to grow the strain within activated sludge at a wastewater treatment plant, or even injecting the bacteria directly into the soil or groundwater of a contaminated site, a process called bioaugmentation. 

“In wastewater- activated sludge systems, you could accelerate removal of undesired compounds by adding a specific strain to the existing bacterial consortium in the treatment plants,” Aga says. “Bioaugmentation is a promising method that has not yet been explored for PFAS remediation in the environment.”

Carbon-Fluorine Bonds: PFAS molecules have extremely strong carbon-fluorine bonds. These tough bonds make the chemicals resistant to natural breakdown, earning them the name "forever chemicals". [1, 2, 3, 4]

Specialized Strains: Recent studies show specific bacteria like Labrys portucalensis F11 [0.6.1 |0.6.15], Acidimicrobium sp. strain A6, and certain Pseudomonas species can target these compounds. [1, 2, 3]

Defluorination: These microbes use special enzymes to detach fluorine atoms and metabolize the remaining carbon for energy [0.6.1 |0.6.15]. [1, 2, 3]

Bioaccumulation: Some human gut bacteria do not destroy PFAS, but they can absorb and trap the chemicals inside their cells to help excrete them

Jonathon Lundgren: 1000 farms project: Regenerative agriculture can feed the world while sequestering carbon co2!

 https://www.youtube.com/watch?v=-_mtHQSVx2g

https://www.youtube.com/watch?v=_8O1dx5TdXI 

 https://www.youtube.com/watch?v=AOVlMaZGup0&t=58s

https://www.youtube.com/watch?v=VEIzWGKbecc 

https://www.youtube.com/watch?v=SRQ1kKNNXis 

https://www.rockefellerfoundation.org/initiatives/regenerative-agriculture/ 

 

Just ordered Alexander P. Anderson Minnesota farmer-botantist "short stories/poetry" book from 1941 (inventor of Puffed Rice!!)

 https://www.featherstonefarm.com/our-story

they sell at our local co-op - gotta try some of their organic corn just now harvested!  

https://www.mnhs.org/mnopedia/search/index/person/anderson-alexander-p-1862-1943

I just ordered his 1941 Minnesota Botany book for $10 from Dallas Halfprice books.

Anderson, Alexander P. The Seventh Reader: Short Stories with Some Verse. Caldwell, Idaho: Caxton Printers, 1941.

The cereal also gained attention as “food shot from guns of peace.”

Online articles from the Smithsonian Institution and the New York Botanical Garden explain:

In 1902, Alexander Anderson received a patent for “a dry method of swelling starch materials of all kinds to render them porous, thereby enhancing their nutritive value and rendering them more readily and completely digested.” The following year he signed an agreement with Quaker Oats to begin large-scale production of puffed rice and wheat cereals. (Source: “Alexander Anderson and the Cereal Shot from Guns” at https://invention.si.edu/alexander-anderson-and-cereal-shot-guns.)

Prior to being marketed as a cereal, Anderson’s puffed rice was promoted as a confection at the 1904 St. Louis World’s Fair. Cannon-like cylinders discharged downpours of puffed rice in a giant cage, wowing the crowds, who purchased packages of the novel treat. (Source: “Breakfast in a Blast: The Invention of Puffed Cereal at NYBG” at https://www.nybg.org.)

Then, the cannons stopped firing. In April 2019, Mae’s Food Blog, posted “The Quaker Oats Company Has Stopped Puffing!” (Read more at https://maefood.blogspot.com/2019/04/the-quaker-oats-company-has-stopped.html.)

Poof!

Quaker Puffed Rice--and Puffed Wheat--disappeared.

The High Protein Diet: Bromelain supplements to hydrolyze a high protein Pea powder diet via biopeptide cleavage

 

 https://pmc.ncbi.nlm.nih.gov/articles/PMC8757916/

Organic dry pea (Pisum sativum L.) biofortification for better human health

 OK looks like I need to get Bromelain tablets to mix in and let it ferment. Fascinating. So one half  of a Bromelain enzyme a day. Not bad.  So I'll mix one capsule for a day's worth of pea powder. hahaha. 

Protein Hydrolysate of Green Peas with Bromelain (PHGPB) is a bioactive peptide compound created by breaking down green pea protein using the pineapple stem enzyme bromelain.  Bromelain is a proteolytic enzyme (protease) derived from pineapples that breaks peptide bonds in proteins,...  Bromelain is a protein-digesting enzyme mixture derived from the stem, fruit, and juice of the pineapple plant. It has a centuries-long history of being used to treat medical ailments, primarily throughout Central and South America.

 

 

peptide derived from the bromelain hydrolysis of Indonesian green peas proteins (PHGPB, 25.71% protein, 9.28% fat) showed the lowest levels of urea and creatinine in cisplatin-induced female Wistar rats (19).... Bromelain 10% (w/v) was added to each solution (25) and then left for 72 hr (26) on a stirrer at room temperature (25–30 ^°C) (27).... Mixing bromelain with pea powder to create a protein hydrolysate typically requires 1 to 4 hours of reaction time at an optimal temperature of 50°C to 55°C (122°F–131°F) and a neutral-to-slightly-alkaline pH (pH 6.8 to 8.0), though some cold or room-temperature protocols extend this up to 24–72 hours.... Legumin or vegetable casein is commonly found in peas, lentils, vetches, and other legumes; it is soluble in water, dissolves in very weak acids and alkalis, and is not coagulated by heat (33). This fact shows that peas possess a good nutritional content for a balanced diet and may even prevent degenerative diseases, such as type II diabetes and cardiovascular disease (34)... bromelain enzyme concentration of 10% yields the highest DH in Nyamplung seed protein (Calophyllum inophyllum) (25). The lowest levels of urea and creatinine that were validated by high super oxide dismutase (SOD) activity and atrial natriuretic peptide (ANP) level were obtained in the 200 mg/day PHGPB treatment.

Googlescholar says it's not just "protein powder" but a "high protein" diet.  

"Kidneys from diseased rats given pea protein compared with casein were more enlarged and had higher fluid content and cyst volumes, despite growing better and having lower serum creatinine and renal chemokine receptor 2 levels, and similar levels of renal fibrosis. Conclusion: Not all plant proteins are equally protective in experimental kidney disease and associated cardiac hypertrophy."

https://onlinelibrary.wiley.com/doi/abs/10.1002/mnfr.201000558

oops! 

a high-protein diet (protein comprising 25% of calories) increased eGFR by 3.8 ml/min per 1.73 m² after 6 weeks compared with a lower protein diet (protein comprising 15% of calories).^9,10 In the early stages, glomerular hyperfiltration occurs as a rise in GFR, in proteinuria, or both, but may result in a loss of kidney function over time

https://pmc.ncbi.nlm.nih.gov/articles/PMC7460905/

The Effects of High-Protein Diets on Kidney Health and Longevity

Kidney therapeutic potential of peptides derived from the bromelain hydrolysis of green peas protein

M Hidayat, S Prahastuti, DU Riany… - Iranian journal of …, 2019 - pmc.ncbi.nlm.nih.gov

… the renal function of cisplatin-induced rats to produce a hydrolysate protein that can improve
kidney … hydrolysis of Indonesian green peas proteins (PHGPB, 25.71% protein, 9.28% fat) …

we found that protein hydrolysate of green peas origin of Indonesia hydrolysed by bromelain (PHGPB) showed improve kidney function in cisplatin-induced nephropathy rats. In this study, we investigated the effect of PHGPB to obtain effective dose that exerts a therapeutic effect on chronic kidney disease (CKD) based on reducing urea and creatinine levels and to elucidate its mechanism of action.

. Higher PHGPB dose corresponds to better effect on kidney function by increasing antioxidant activity and ANP levels in gentamycin-induced Wistar rats.

A Canadian study stated that the hydrolysate protein of yellow peas (Pisum sativum L.) alleviates high blood pressure and CKD. Pea protein hydrolysate (PPH) was used as a potential modulator of the renin–angiotensin system (RAS). PPH reduces blood pressure by increasing the levels of cyclooxygenase-1 (COX-1) in renal tissues (2). Hydrolysate proteins are amino acid mixtures obtained from the degradation of hydrolyzed proteins using acids, bases, or proteolytic enzymes to produce peptides and small molecules with high solubility (3).Enzymatic protein hydrolysis improves the function and nutrients of protein sources; it can contain large amounts of efficient peptides without toxic by-products or amino acids (4, 5).

Hydrolysis of green peas proteins produces antioxidant, anti-inflammatory, and hypolipidemic peptides that can be used as supportive therapy in cardiovascular disease (6). Such peptides also display antimicrobial, antihypertensive, immunomodulatory, and anticancer activities (7, 8).

follow up study

Cleavage of protein polypeptide chains generates short-chain peptides, some of which could exhibit biological activities. Such cleavage, as a result of hydrolysis reactions, could occur during the natural digestion in human gastrointestinal tract but also could be achieved by food processing techniques such as enzymatic hydrolysis, microbial fermentation, and in vitro digestion simulation. For pea protein, the bioactivities of the resultant peptides, or hydrolysates, mainly include antioxidant, antimicrobial, antihypertensive, and antidiabetic characteristics as reported in recent works.

 https://www.sciencedirect.com/science/article/abs/pii/S2214799322001461

purified organic pea protein powder (such as that produced by PURIS) is separated from the hull and starch during processing, meaning it functions as a protein isolate rather than a hull fiber product. PURIS separates the components into distinct streams, offering pea protein isolates and separate organic pea hull fiber (OPF-Hulls) as entirely different ingredients. [1, 2]

Product Differences

PURIS Organic Pea Protein: Highly purified (over 80% protein content) with most fiber, starch, and hull components removed.
 Green peas (Pisum sativum) were hydrolyzed by bromelain from pineapple juice to obtain PHGPB.  Bromelain was isolated from pineapple juice (Ananas sativus)
 

I've been off Refined Sugar for a month now - feels awesome

 https://sugarscience.ucsf.edu/

https://www.youtube.com/watch?v=pvh5B1rHOtw 

I stopped getting "internal tingling" from the yin qi neutralizing the free radicals created by sugar - a least almost stopped it. Way way less - almost all gone. Pretty wild.

 

I ordered 44 lb bulk Minnesota-grown Organic Pea Powder at $8.30 per pound: Puris

 So if you buy organic pea powder in a bottle it is 1.5 lbs at $17 a pound. So I'm getting it for half price by buying it bulk. I plan to eat a LoT of this stuff - to increase protein intake.  "You pay extra for a product that mixes into liquids instantly without cooking or blending....Yes, organic pea protein powder is a healthy, safe, and high-quality plant-based supplement. It is rich in iron and essential amino acids, easy to digest,"

 4 level tablespoons: A standard measuring tablespoon holds roughly 7.5 grams of protein powder.

1/4 cup of organic pea powder has 2% of calcium also with 21 grams of protein... "You digest and absorb about 21 to 23 grams of protein from a 30-gram scoop of typical organic pea protein powder, given its high digestibility rate of roughly 90% to 95%."

https://www.startribune.com/puris-pea-protein-shakes/601866252

 Just ordered a 44 lb bag of organic peas powder grown in Minnesota. hahaha. https://www.puris.com/about/regenerative-agriculture

https://www.youtube.com/watch?v=PIGmWFMS-34

Supposed to be "less gritty" than normal pea protein powder....

"No glyphosate residue"!!! hahaha

Let’s start with the flavor: it’s amazing. Simply the best of any protein powder – not only pea protein, any. Then, it’s perfectly powderized, so it blends to perfection... It isn't over powered with sweetness or fake sugars like other protein powders. It's very versatile.... Not an ounce of chalkiness taste as in a lot of other non-flavored pea protein powders.

Their website was cheaper than Jeff Bezos' website! Wow - you don't see that very often...

 https://www.puris.com/ingredients/pea-proteins/p870ls/

https://www.youtube.com/shorts/Xrsx28LKArI

I used to order bulk organic lentils from the co-operative but they fired their "bulk order" lady (who lived in my town - cutting co-op costs!). Now they have some online form - and I tried submitting it and I never heard back. I then asked again and left a note - they took it down. Never heard back. Then they hire a "customers experience diirector" hahahahahahhahahaha. I kid you not! Just some administrator who has surveys or something stupid.

I used to order 25 lb bags of organic rice and organic lentils. Wow - this will be huge but I'll keep it in my blue barrel ...cover it up...store in cool but not freezing place....load up on protein while losing weight...no whey digestion problems...

 

Military TR-3B triangle crafts are from Nazi-CIA collaboration, NOT E.T. alien craft

Yeah John Keel wrote cool books and gave cool talks but I don't trust him.

Gray Barker: An eccentric writer and publisher from West Virginia who wrote They Knew Too Much About Flying Saucers (1956), popularizing the "Men in Black". Later evidence showed he treated UFO lore largely as entertainment and commercial enterprise. [1, 2, 3]
James Moseley: The publisher of Saucer News, a sharp-witted debunker-turned-participant in the UFO scene known for his humor and elaborate lecture circuit props. [1, 2, 3]
John Keel: Author of The Mothman Prophecies, who was closer to a Fortean investigator than a nuts-and-bolts ufologist, though he frequently interacted with—and was targeted by—Barker and Moseley's pranks. [1, 2]

Collaborations and Hoaxes

The Lost Creek UFO Film: In 1966, Barker and Moseley staged a deliberate UFO hoax. They filmed a ceramic saucer model ("boogie" saucer) held on a fishing pole in front of a car in Lost Creek, West Virginia. Moseley used the footage in lectures and television appearances as a gag prop, while Barker sold copies through his mail-order service. [1]
Pranks and Retribution: The trio and their peers maintained a tight, cynical inner circle behind the scenes, frequently pulling elaborate satirical stunts on each other and testing the credulity of the broader public UFO community

Barker wrote an article about the Flatwoods monster and flying saucers and submitted it to FATE Magazine, a magazine devoted to paranormal phenomena.  According to Wikipedia, FATE was co-founded in 1948 by Raymond A. Palmer (editor of Amazing Stories) and Curtis Fuller.  Still in publication today, FATE is now the longest-running magazine devoted to the paranormal.

For Barker, that was fate indeed!  His article, “The Monster and the Saucer,” was accepted and published in January 1953.  
https://news.lib.wvu.edu/2021/05/25/gray-barker-and-the-men-in-black-they-knew-too-much-about-flying-saucers/

Gray Barker’s book, The Silver Bridge, linked the collapse of the Silver bridge in Point Pleasant with the appearance of the Mothman,

https://evergreenreview.com/read/it-came-from-west-virginia/

But, as documented in Gabriel Mckee’s The Saucerian: UFOs, Men in Black, and the Unbelievable Life of Gray Barker (The MIT Press, 2025), scaring people would become his meal ticket—and his passion. Barker would grow up to become one of the leading publishers of literature on flying saucers, alien life forms, and the conspiracies that covered them up. And he did it in ALL CAPS.

That Barker hailed from Appalachia, rich in the folklore of angels, devils and tricksters, was not incidental. Science fiction merely updated these archetypes for the atomic age.

In the postwar era, the leading science fiction magazine was Raymond Palmer’s Amazing Stories, whose “hard science” readers were dismayed when he started publishing stories by Richard Shaver, beginning with 1945’s “I Remember Lemuria!” Shaver claimed that below the surface of our planet lived two warring clans of subterranean robots: the Dero, or detrimental robots, who tormented surface people with ray guns, and the benevolent but ineffectual Tero, or integrative robots. Thus was born “The Shaver Mystery.” Amazing Stories even received a letter from someone who claimed he’d fought the Dero in WWII. The lines between hard sci-fi and tales of the paranormal were starting to blur. And it was in that shifting middle ground where Barker would make his home.

  John Keel, who gave the Men in Black their name. Keel was also an adept hoaxer, although susceptible to being hoaxed himself. His bestseller The Mothman Prophecies, published nearly a decade later (and which became a 2002 movie starring Richard Gere), is strewn with the residue of hoaxes perpetrated on Keel for over a year by Barker and Moseley, in retribution for a prank Keel pulled on Barker.

I think that military space travel was more advanced soon after World War II due to the Nazi-CIA collaboration....

 More National UFO Center "triangle" sightings:

It had a large blinking light at its center on the bottom and three constant glowing lights at each point on the bottom. My camera is shaky so they look like they're blinking. They're not. Only the center light strobed consistently. You could hear a faint low hum like a jet only much quieter. You cannot hear it on the video. It flew very eerily, low, slow, and appeared bigger than our typical fighter jets. It seemed to fly in a somewhat straight direction moving from West to East/Southeast. I've lived in the rural country all my life and I love star gazing and watching aircraft/satellites. We have a nearby municipal airport and military jets fly by often (old and new). I've NEVER seen this before. It was NOT in a V shape or T shape, it was a complete triangle.  

.....

 https://nuforc.org/sighting/?id=200055\

Fantastic video of the craft on that link!! But it's way farther than what I saw. My sighting was about 50 feet from me.

Here is someone who similarly had the triangle craft fly low over their yard just like me!!

could hear a wooshing sound like air passing by. I saw the craft lights but the aircraft was unable to be seen. It looked like a backwards triangle with two evenly spaced white lights at the front and one white light at the back, that did not blink. Just stationary white lights. I only heard the craft when it was slowly moving over my backyard. It did not sound like a jet. It was very strange and before it came over my yard and I looked up, my back solar light started growing brighter then dimmed before it glided over my home and again I could not see the craft only the 4 lights in a backwards triangle.

 https://nuforc.org/sighting/?id=199372

Yeah it's definitely a "metal" body to the TR-3B

there I saw above the tree line, but it wasn’t right above me it was a few miles away but In the air just hovering. It was clear as day a triangle shaped craft, the top was a grayish blue metallic color and the bottom was just black… as I was driving I couldn’t see because the trees were blocking it but as I kept continuing more on the highway I saw it again in the same spot I clearly knew that was not an aircraft. It was very large. It had to be the size of a car even bigger. It was not directly above me though.

 https://nuforc.org/sighting/?id=199278

a triangular UAP appeared from the left of us, it was telephone pole high and was slowly spinning in a circle while moving to the right slowly. It had no sound to the craft and it had lights on each side of it. It wasn’t going fast and there was cars behind us as well, atleast 5.

At first glance I literally thought it was a plane that lost power and was gliding down. But after observing longer I noticed it was rotating and hovering to the right, it was rotating and hovering slowly. It had a green light on one corner of it and a red one on the other corner. It came from behind trees on the left side of the road &’ ended up headed towards fields on the right.

What’s weird was the lights were very visible like obvious what colors they were but it’s like the lights were just there. The lights didn’t travel anywhere. It was as high as the telephone poles and right in front on my windshield view. Me and my daughter drew images of what the craft looked like to us, I drew mine in the living room, she drew hers in her room. Our drawings matched with how to craft looked. My boyfriend claims he heard it making a buzzing sound.

Yep it makes a low humming noise that you can hear if you are that close to it!

 https://nuforc.org/sighting/?id=199205

Here we have TWO triangles flying side by side and then a military helicopter follows up...

https://nuforc.org/sighting/?id=199107

sometimes the Triangle craft is seen to "vanish" after becoming a very bright light:

Three bright lights, red blue and green on the corners of a triangle four or five bright white lights in the center
It was a triangle shaped ship, but all I could see were lights on the bottom three blue green and red. It was flying northeast. I only saw it for about I wanna say 10 seconds flying north east then it stopped bright lights underneath white ones four or five they be came super bright lit up the sky for just a second went dim, and then the whole ship shot straight up out into space.
https://nuforc.org/sighting/?id=198827

I saw a huge triangular grayish black craft pass over our houses. The bulk of the craft was completely over the neighbor's house and extended partly over our house. It was moving very slow, I would guess about 15-25 mph. It was flying very low probably about 50-75 feet above the tree line. As it passed over, I could hear a low humming sound.

https://nuforc.org/sighting/?id=197226

 

 It was in the sky it was triangle shape and not high in the sky but not too low, it was hovering and a beam came out of it

Then a beam of light came out from it idk how to explain but the light was so damn bright like it was crazy

Blue/red lights, black triangle no sound darts with a light trail behind, moves up, down, horizontal etc

Blue/red lights, black triangle no sound darts with a light trail behind, moves up, down, horizontal. Then completely moves so quickly it's gone.